alexa Profiling the chlorogenic acids of Rudbeckia hirta, Helianthus tuberosus, Carlina acaulis and Symphyotrichum novae-angliae leaves by LC-MS(n)


Journal of Chemical Biology & Therapeutics

Author(s): Jaiswal R, Deshpande S, Kuhnert N

Abstract Share this page

INTRODUCTION: Chlorogenic acids (CGAs) are a family of esters formed between quinic acid and certain cinnamic acids, most commonly caffeic, p-coumaric and ferulic acid. They show a variety of biological activities like antioxidant, anti-inflammatory, anti-HIV, anti-HBV and inhibition of mutagenesis and carcinogenesis, and are considered to be beneficial to human health. We have selected well-established plants of Asteraceae family and these are used in folk medicines as antivirals. OBJECTIVE: Investigating the CGA profile of anti-viral herbal remedies, we aim at identifying selected chlorogenic acids commonly encountered in these plants in order to identify suitable candidates for anti-viral screening. It should be noted that neuroaminidase inhibitors, recently introduced into the clinical practice as Tamiflu or Relenza, bear a remarkable structural similarity to CGAs, suggesting potentially a similar mode of action. METHODOLOGY: Methanolic extracts were used for LC-MS analysis. The LC equipment with DAD detector was interfaced with a high-resolution MicroTOF mass spectrometer for molecular formula assignment and an ion trap mass spectrometer for assignment of CGA regiochemistry based on characteristic fragmentation patterns. For the identification of cis cinnamoylquinic acids, extracts were treated with UV-irradiation. RESULTS: A series of CGAs were identified by their tandem mass spectra and retention times. We also found that diacyl CGAs containing cis isomer of cinnamic acids have the same tandem MS spectra as those containing the trans isomer. CONCLUSION: In this study, all the CGAs present in the leaves of Rudbeckia hirta, Helianthus tuberosus, Carlina acaulis and Symphyotrichum novae-angliae were identified qualitatively to their regioisomeric level without any purification or isolation, and assignment was based on their LC-MS(n) behaviour and the change in the intensity after the UV-irradiation. A series of CGAs common to these anti-viral herbal remedies was identified, which will in the future be selected for further detailed biological screening.

  • To read the full article Visit
  • Subscription
This article was published in Phytochem Anal and referenced in Journal of Chemical Biology & Therapeutics

Relevant Expert PPTs

Relevant Speaker PPTs

Recommended Conferences

Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri, Food, Aqua and Veterinary Science Journals

Dr. Krish

1-702-714-7001 Extn: 9040

Clinical and Biochemistry Journals

Datta A

1-702-714-7001Extn: 9037

Business & Management Journals


1-702-714-7001Extn: 9042

Chemical Engineering and Chemistry Journals

Gabriel Shaw

1-702-714-7001 Extn: 9040

Earth & Environmental Sciences

Katie Wilson

1-702-714-7001Extn: 9042

Engineering Journals

James Franklin

1-702-714-7001Extn: 9042

General Science and Health care Journals

Andrea Jason

1-702-714-7001Extn: 9043

Genetics and Molecular Biology Journals

Anna Melissa

1-702-714-7001 Extn: 9006

Immunology & Microbiology Journals

David Gorantl

1-702-714-7001Extn: 9014

Informatics Journals

Stephanie Skinner

1-702-714-7001Extn: 9039

Material Sciences Journals

Rachle Green

1-702-714-7001Extn: 9039

Mathematics and Physics Journals

Jim Willison

1-702-714-7001 Extn: 9042

Medical Journals

Nimmi Anna

1-702-714-7001 Extn: 9038

Neuroscience & Psychology Journals

Nathan T

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

John Behannon

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

1-702-714-7001 Extn: 9042

© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version