alexa Prolonged hypoxia accelerates the posttranscriptional process of collagen synthesis in cultured fibroblasts.
Molecular Biology

Molecular Biology

Journal of Cytology & Histology

Author(s): Horino Y, Takahashi S, Miura T, Takahashi Y

Abstract Share this page

Abstract Molecular oxygen is essential for metazoan life. Hypoxia, a reduced oxygen condition, induces systemic and cellular responses for acclimation to the limited oxygen availability. Multicellularity of metazoans is maintained on extracellular matrices. Previously, we demonstrated that acute hypoxia up-regulated the prolyl 4-hydroxylase alpha(I) subunit, the rate-limiting subunit for the hydroxylation of proline residues of procollagens (Y. Takahashi, S. Takahashi, Y. Shiga, T. Yoshimi, and T Miura, 2000, J. Biol. Chem., 275, 14139-14146). The formation of hydroxyproline is an essential posttranscriptional process for stabilization of the helical trimer of procollagen polypeptides at physiological temperature. In this present study, we cultured fetal rat lung fibroblasts for up to 9 days and examined the effects of prolonged hypoxia on the level of procollagen mRNA in the cells and the posttranscriptional steps of collagen synthesis. Hypoxia accelerated the deposition of collagen molecules. These enhancements in hypoxic cultures were observed with or without ascorbic acid in the culture medium. The steady-state level of procollagen alpha1(I) mRNA was not affected by the prolonged hypoxia. In contrast, the mRNA and protein levels of the prolyl 4-hydroxylase alpha(I) subunit were increased by hypoxic culture under both ascorbic acid-sufficient and -deficient conditions. These results suggest that hypoxic enhancement of the posttranscriptional step of collagen synthesis contributed to the accelerated deposition of collagen fibrils.
This article was published in Life Sci and referenced in Journal of Cytology & Histology

Relevant Expert PPTs

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri, Food, Aqua and Veterinary Science Journals

Dr. Krish

[email protected]

1-702-714-7001 Extn: 9040

Clinical and Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemical Engineering and Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001 Extn: 9040

Earth & Environmental Sciences

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

General Science and Health care Journals

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics and Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001 Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Informatics Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Material Sciences Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Mathematics and Physics Journals

Jim Willison

[email protected]

1-702-714-7001 Extn: 9042

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001 Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

John Behannon

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001 Extn: 9042

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords