Author(s): Kim CH, Axup JY, Schultz PG, Kim CH, Axup JY, Schultz PG, Kim CH, Axup JY, Schultz PG, Kim CH, Axup JY, Schultz PG
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Abstract The site-specific incorporation of unnatural amino acids with orthogonal chemical reactivity into proteins enables the synthesis of structurally defined protein conjugates. Amino acids containing ketone, azide, alkyne, alkene, and tetrazine side chains can be genetically encoded in response to nonsense and frameshift codons. These bio-orthogonal chemical handles allow precise control over the site and stoichiometry of conjugation, and have enabled medicinal chemistry-like optimization of the physical and biological properties of protein conjugates, especially the next-generation protein therapeutics. Copyright © 2013. Published by Elsevier Ltd.
This article was published in Curr Opin Chem Biol
and referenced in Immunotherapy: Open Access