alexa Protein Phosphorylation in Amyloplasts Isolated from Suspension-Cultured Cells of Sycamore (Acer pseudoplatanus L.).
Environmental Sciences

Environmental Sciences

Journal of Biodiversity, Bioprospecting and Development

Author(s): Macherel D, Viale A, Akazawa T, Macherel D, Viale A, Akazawa T

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Abstract Highly purified amyloplasts were isolated from cultured cells of sycamore (Acer pseudoplatanus L.). Incubation of amyloplasts with [gamma-(32)P]-ATP resulted in the labeling of more than ten polypeptides. Pulsechase experiments showed the reversibility of the process with some but not all of the polypeptides. The phosphorylation reaction of one polypeptide, M(r) 100, was shown to be calcium dependent. Although exogenously added pig brain calmodulin had no effect, the calmodulin antagonist W-7 strongly inhibited phosphorylation of the 100 kilodaltons polypeptide. The presence of endogenous calmodulin, about 1 to 3 micrograms per milligram protein, in the amyloplast preparation was estimated by activation of phosphodiesterase in vitro.
This article was published in Plant Physiol and referenced in Journal of Biodiversity, Bioprospecting and Development

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