Author(s): Ohyama K, , Huy NT, Yoshimi H, Kishikawa N,
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Abstract Immune complexes (ICs) are the direct and real-time products of humoral immune responses. The identification of constituent foreign or autoantigens within ICs might bring new insights into the pathology of infectious diseases. We applied immune complexome analysis of plasma to the study of Chagas disease caused by Trypanosoma cruzi. Twenty sero-positive plasma samples including cardiac and/or megacolon determinate patients (n=11) and indeterminate (n=9) were analyzed along with 10 seronegative individuals to characterize the antigens bound to circulating ICs. We identified 39 T. cruzi antigens and 114 human auto-antigens specific to Chagas patients. Among those antigens, two T. cruzi antigens (surface protease GP63, glucose-6-isomerase) and six human auto-antigens (CD180 antigen, ceruloplasmin, fibrinogen beta chain, fibrinogen beta chain isoform 2 preprotein, isoform gamma-A of fibrinogen γ chain, serum paraoxonase) were detected in more than 50\% of the patients tested. Human isoform short of complement factor H-related protein 2 and trans-sialidase of T.cruzi were more frequently found in the indeterminate (5/9 for both) compared to in the determinate Chagas (0/11, P = 0.046 for human, 1/11, P = 0.0498 for T.cruzi). The immune complexome could illustrate the difference of immune status between clinical forms of chronic Chagas disease. This article is protected by copyright. All rights reserved. This article is protected by copyright. All rights reserved.
This article was published in Parasite Immunol
and referenced in Journal of Antivirals & Antiretrovirals