alexa Purification, crystallization and structure determination of native GroEL from Escherichia coli lacking bound potassium ions.
Chemistry

Chemistry

Journal of Physical Chemistry & Biophysics

Author(s): Kiser PD, Lodowski DT, Palczewski K

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Abstract GroEL is a member of the ATP-dependent chaperonin family that promotes the proper folding of many cytosolic bacterial proteins. The structures of GroEL in a variety of different states have been determined using X-ray crystallography and cryo-electron microscopy. In this study, a 3.02 A crystal structure of the native GroEL complex from Escherichia coli is presented. The complex was purified and crystallized in the absence of potassium ions, which allowed evaluation of the structural changes that may occur in response to cognate potassium-ion binding by comparison to the previously determined wild-type GroEL structure (PDB code 1xck), in which potassium ions were observed in all 14 subunits. In general, the structure is similar to the previously determined wild-type GroEL crystal structure with some differences in regard to temperature-factor distribution.
This article was published in Acta Crystallogr Sect F Struct Biol Cryst Commun and referenced in Journal of Physical Chemistry & Biophysics

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