alexa Purification of binder of sperm protein 1 (BSP1) and its effects on bovine in vitro embryo development after fertilization with ejaculated and epididymal sperm.


International Journal of Advancements in Technology

Author(s): RodrguezVillamil P, HoyosMarulanda V, Martins JA, Oliveira AN, Aguiar LH,

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Abstract The present study evaluated functional aspects of binder of sperm 1 (BSP1) in the bovine species. In a first experiment, cumulus-oocyte complexes (n = 1274) were incubated with frozen-thawed ejaculated sperm (18 hours) in Fert-TALP medium containing: heparin, 10, 20, or 40 μg/mL BSP1. Heparin followed by gelatin affinity chromatography was used for purification of BSP1 from bovine seminal vesicle fluid. With ejaculated sperm, cleavage rates were similar when Fert-TALP medium was incubated with heparin (74.1 ± 2.7\%), 10 μg/mL BSP1 (77.8 ± 3.1\%), or 20 μg/mL BSP1 (74 ± 2.0\%). Day-7 blastocyst rates were equivalent after incubations with heparin (40.8 ± 5.0\%) and 10 μg/mL BSP1 (34.1 ± 4.4\%), but reduced after 20 μg/mL BSP1 (22.4 ± 2.9\%) and 40 μg/mL BSP1 (19.3 ± 4.1\%; P < 0.05). In the second experiment, cumulus-oocyte complexes (n = 1213) were incubated with frozen-thawed cauda epididymal sperm (18 hours) in Fert-TALP medium containing: no heparin, heparin, 10, 20, or 40 μg/mL. Cleavage and blastocyst rates were similar after treatments with heparin (68.5 ± 1.3\% and 24.7 ± 3.2\%, respectively) or without heparin (65.5 ± 1.8\% and 27.3 ± 1.6\%, respectively). Cleavage was higher after treatment with any BSP1 concentrations (74.2 ± 2.7\%-79.0 ± 1.1\%) than without heparin (P < 0.05). Also, cleavage was better after Fert-TALP medium incubation with 40 μg/mL BSP1 (79.0 ± 1.1\%) than with heparin (68.5 ± 1.3\%; P < 0.05). Embryo development was higher (P < 0.05) after treatment with 20 μg/mL BSP1 (35.6 ± 2.5\%) and 40 μg/mL (41.1 ± 2\%) than after incubations with heparin (24.7 ± 3.2\%) or without heparin (27.3 ± 1.6\%). Interestingly, BSP1 did not cause reductions in blastocyst rates after fertilization with epididymal sperm, as observed with ejaculated sperm. On the basis of immunocytochemistry, there was BSP1 binding to frozen-thawed ejaculated but not to epididymal sperm. Also, anti-BSP1 reaction remained on ejaculated sperm (as expected) and appeared on epididymal sperm after incubation with purified BSP1. Acrosome reaction of ejaculated and epididymal sperm was induced after incubation with purified BSP1 as well, indicating an effect of BSP1 on capacitation. In conclusion, purified BSP1 from bull seminal vesicles was able to bind to and induce capacitation of ejaculated and epididymal sperm. Also, BSP1 added to fertilization media and allowed proper cleavage and embryo development, with the effects being modulated by previous exposure or not of spermatozoa to seminal plasma. Copyright © 2016 Elsevier Inc. All rights reserved. This article was published in Theriogenology and referenced in International Journal of Advancements in Technology

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