Chemical Engineering

Chemical Engineering

Journal of Chromatography & Separation Techniques


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Annona squamosa Linn is a multipurpose tree with edible fruits & a source of the medicinal & industrial products. Annona squamosa Linn is used as an antioxidant, antidiabetics , hepatoprotective, cytotoxicactivity, genetoxicity, antitumor activity, antilice agent. The leaves of Annona squamosa contains valuable quantity of ascorbic acid and tannins. Vitamin -C, chemically known as ascorbic acid, is an important component of a healthy diet. The history of vitamin -C revolves around the history of the human disease scurvy, probably the first human illness to be recognized as a deficiency disease. Its symptoms include exhaustion, massive hemorrhaging of flesh and gums, general weakness and diarrhea. Vitamin C (ascorbic acid, AA) is a water -soluble organic compound involved in many biological processes . Keywords: Ascorbic acid, Annona squamosa , HPLC method , UV spectrophotometric . A number of methods are available and are being developed for the extraction and characterization of As corbic acid from plants . This article deals with extraction and to develop a simple, efficient, reliable and cost- effective high performance liquid chromatographic (HPLC) and UV spectrophotometric method s for the quantization of vitamin C in leaves of A.sq uamosa . The method is simple, rapid and has high specificity to ascorbic acid. Each analysis require less than 5 min. Qualitative estimation was carried out by thin layer chromatographic (TLC) method. HPLC separation was performed on a Cyber Lab C -18 colum n (250 x 4.0 mm, 5μ) using Water(A) and 0.34% o -phosphoric acid (B) using an isocratic elution as follow: 0 –30 min, 40%A –80% A, 60%B –20% B. The flow rate was 1.5 mL/min, column temperature 25°C, the injection volume was 25μl and UV detection was effected a t 266 nm

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This article was published in International Journal of Pharmacy and Pharmaceutical Sciences and referenced in Journal of Chromatography & Separation Techniques

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