Author(s): Reischer GH, Lemmens M, Farnleitner A, Adler A, Mach RL
Abstract Share this page
Abstract A new real-time PCR based method was developed for the species-specific detection, identification and quantification of Fusarium graminearum in planta. It utilizes a TaqMan hybridisation probe targeting the beta-tubulin gene and a plasmid standard. The assay is highly specific giving no product with DNA of closely related species. It is very sensitive, detecting down to five gene copies per reaction, and is able to produce reliable quantitative data over a range of six orders of magnitude.
This article was published in J Microbiol Methods
and referenced in Journal of Plant Pathology & Microbiology