alexa Quantification of Fusarium graminearum in infected wheat by species specific real-time PCR applying a TaqMan Probe.


Journal of Plant Pathology & Microbiology

Author(s): Reischer GH, Lemmens M, Farnleitner A, Adler A, Mach RL

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Abstract A new real-time PCR based method was developed for the species-specific detection, identification and quantification of Fusarium graminearum in planta. It utilizes a TaqMan hybridisation probe targeting the beta-tubulin gene and a plasmid standard. The assay is highly specific giving no product with DNA of closely related species. It is very sensitive, detecting down to five gene copies per reaction, and is able to produce reliable quantitative data over a range of six orders of magnitude. This article was published in J Microbiol Methods and referenced in Journal of Plant Pathology & Microbiology

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