alexa Quantitative phosphoproteome analysis using a dendrimer conjugation chemistry and tandem mass spectrometry.
Immunology

Immunology

Journal of Clinical & Cellular Immunology

Author(s): Tao WA, Wollscheid B, OBrien R, Eng JK, Li XJ,

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Abstract We present a robust and general method for the identification and relative quantification of phosphorylation sites in complex protein mixtures. It is based on a new chemical derivatization strategy using a dendrimer as a soluble polymer support and tandem mass spectrometry (MS/MS). In a single step, phosphorylated peptides are covalently conjugated to a dendrimer in a reaction catalyzed by carbodiimide and imidazole. Modified phosphopeptides are released from the dendrimer via acid hydrolysis and analyzed by MS/MS. When coupled with an initial antiphosphotyrosine protein immunoprecipitation step and stable-isotope labeling, in a single experiment, we identified all known tyrosine phosphorylation sites within the immunoreceptor tyrosine-based activation motifs (ITAM) of the T-cell receptor (TCR) CD3 chains, and previously unknown phosphorylation sites on total 97 tyrosine phosphoproteins and their interacting partners in human T cells. The dynamic changes in phosphorylation were quantified in these proteins. This article was published in Nat Methods and referenced in Journal of Clinical & Cellular Immunology

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