alexa Radiochemical purity and particles number determinations of modified 99m Tc-macroaggregated albumin
Chemical Engineering

Chemical Engineering

Journal of Chromatography & Separation Techniques


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A new procedure for the aggregation of human albumin and 99m Tc-labelling of the prepared macroaggregated albumin are presented. Simple methods for quantifying of all the radiochemical impurities existing in 99m Tc-MAA were tested. Thus, 85 % methanol was used as the mobile phase in paper and ITL chromatography with Whatman N o 1 and ITLC-SA strips. A system of two solvents (acetone an d 1 M NaCl or 0.9 % NaCl) was used for 3 MM paper, ITLC-SA and ITLC-SG strips and silica gel plates as the stationary phase. Low-voltage paper electrophoresis with Whatman 3 MM paper sheets soaked in barbiturate buffer and the gel chromatography column method (Sephadex G-25) were also applied. Fil- tration through syringe filters, proposed by European and Yugoslav Pharmacopoeia, was performed for comparison. The application of the mentioned tests lead to consistent results for the labelling efficiency (> 98.5 %) and percent radiochemical impurities of 99m Tc-MAA. Determination of the particles number in a counter chamber and their size distribution under a light microscope with a calibrated ocular scale gave the result of 300000 –350000 particles per 1 mg of HA. This confirmed that the human albumin macroaggregates prepared by our new procedure is remarkably improved and convenient for routine diagnostic purposes

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This article was published in Journal of Serbian Chemical Society and referenced in Journal of Chromatography & Separation Techniques

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