alexa Rapid and sensitive detection of physical status of human papillomavirus type 16 DNA by quantitative real-time PCR
Infectious Diseases

Infectious Diseases

Journal of Infectious Diseases & Therapy

Author(s): Nagao S, Yoshinouchi M, Miyagi Y, Hongo A, Kodama J

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A rapid quantitative real-time PCR method was employed to quantify the copy number of E2 and E6 genes for analysis of the physical status of human papillomavirus type 16 (HPV-16) DNA. Significant differences with respect to both copy numbers were found when more than 40% of HPV-16 DNA was integrated with disruption of the E2 gene in an experimental model. The physical status of HPV-16 DNA in 50 clinical samples was exclusively episomal in 21 cases (42%), concomitant in 14 cases (28%), and integrated in 15 cases (30%). The prevalence of integrated and/or concomitant forms of HPV-16 DNA increased with progression of cervical disease. Four of 11 cervical intraepithelial neoplasia involved integrated forms of HPV-16 DNA partially or exclusively. This rapid, sensitive technique is useful in the analysis of the physical status of HPV DNA.

This article was published in Journal of Clinical Microbiology and referenced in Journal of Infectious Diseases & Therapy

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