alexa Rapid detection of anti-Vaccinia virus neutralizing antibodies.
General Science

General Science

Journal of Bioterrorism & Biodefense

Author(s): Kramski M, Drozd A, Lichtfuss GF, Dabrowski PW, Ellerbrok H

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Abstract Increasing infections with Monkeypox and Cowpox viruses pose a continuous and growing threat to human health. The standard method for detecting poxvirus neutralizing antibodies is the plaque-reduction neutralization test that is specific but also time-consuming and laborious. Therefore, a rapid and reliable method was developed to determine neutralizing antibody titers within twelve hours. The new assay measures viral mRNA transcription as a marker for actively replicating virus after incomplete neutralization using real-time PCR. © 2011 Kramski et al; licensee BioMed Central Ltd.
This article was published in Virol J and referenced in Journal of Bioterrorism & Biodefense

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