Author(s): Gimeno E, Castellote AI, LamuelaRavents RM, de la Torre MC, LpezSabater MC
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Abstract A quick and direct method for measuring tocopherols (alpha, beta+gamma and delta) in vegetable oils has been developed using RP-HPLC with UV detection. Previous extraction of tocopherols is not required. The oil is diluted in hexane and an aliquot is mixed with ethanol containing an internal standard (alpha-tocopherol acetate). The chromatographic system consists of an ODS-2 column with a methanol-water mobile phase. Tocopherols are detected at 292 nm in less than 5 min after injection. The method is precise (RSD=2.69\%) and has a high mean recovery (98.14\%).
This article was published in J Chromatogr A
and referenced in Journal of Analytical & Bioanalytical Techniques