Author(s): Stergachis AB, MacLean B, Lee K, Stamatoyannopoulos JA, MacCoss MJ
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Abstract We report a method for high-throughput, cost-efficient empirical discovery of optimal proteotypic peptides and fragment ions for targeted proteomics applications using in vitro-synthesized proteins. We demonstrate the approach using human transcription factors, which are typically difficult, low-abundance targets and empirically derived proteotypic peptides for 98\% of the target proteins. We show that targeted proteomic assays developed using our approach facilitate robust in vivo quantification of human transcription factors.
This article was published in Nat Methods
and referenced in Journal of Carcinogenesis & Mutagenesis