alexa Recent advances in peptide probe-based biosensors for detection of infectious agents.
Microbiology

Microbiology

Journal of Microbial & Biochemical Technology

Author(s): Dover JE, Hwang GM, Mullen EH, Prorok BC, Suh SJ

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Abstract Recent biological terrorism threats and outbreaks of microbial pathogens clearly emphasize the need for biosensors that can quickly and accurately identify infectious agents. The majority of rapid biosensors generate detectable signals when a molecular probe in the detector interacts with an analyte of interest. Analytes may be whole bacterial or fungal cells, virus particles, or specific molecules, such as chemicals or protein toxins, produced by the infectious agent. Peptides and nucleic acids are most commonly used as probes in biosensors because of their versatility in forming various tertiary structures. The interaction between the probe and the analyte can be detected by various sensor platforms, including quartz crystal microbalances, surface acoustical waves, surface plasmon resonance, amperometrics, and magnetoelastics. The field of biosensors is constantly evolving to develop devices that have higher sensitivity and specificity, and are smaller, portable, and cost-effective. This mini review discusses recent advances in peptide-dependent rapid biosensors and their applications as well as relative advantages and disadvantages of each technology. This article was published in J Microbiol Methods and referenced in Journal of Microbial & Biochemical Technology

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