alexa Recombinant production of a peroxidase-protein G fusion protein in Pichia pastoris.
Chemical Engineering

Chemical Engineering

Journal of Chromatography & Separation Techniques

Author(s): Krainer FW, Darnhofer B, BirnerGruenberger R, Glieder A

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Abstract Streptococcal protein G (SpG) binds immunoglobulin G from a broad range of mammalian species with high affinity. Chemical conjugations of SpG to the reporter enzyme horseradish peroxidase (HRP) are commonly used in immunohistochemical applications. However, commercial HRP preparations are typically isolated from horseradish roots as varying mixtures of HRP isoenzymes with different biochemical properties, and chemical conjugation procedures lead to heterogeneous HRP-SpG preparations, partially including inactivated enzyme. A recombinant process allows the production of a well-defined HRP isoenzyme fused to SpG at constant 1:1 stoichiometry in a single step without the need for laborious chemical conjugation. By using state-of-the-art biotechnological tools, we produced a recombinant HRP-SpG fusion protein in Pichia pastoris in bioreactor cultivations. Purified HRP-SpG was tested successfully for functional binding of antibodies from different mammalian serums. Recombinant production of this novel well-defined fusion protein follows quality-by-design principles and facilitates the production of more reliable and cost-effective diagnostic kits. Copyright © 2015 The Authors. Published by Elsevier B.V. All rights reserved. This article was published in J Biotechnol and referenced in Journal of Chromatography & Separation Techniques

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