Author(s): Fitzgibbons JF, Koler RD, Jones RT
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Abstract The minor hemoglobin components, hemoglobin AIa+b and hemoglobin AIc, were measured in the 10\% youngest and 10\% oldest erythrocytes of 15 normal and 14 diabetic subjects. Erythrocyte fractions were obtained by centrifugation in isopyknic concentrations of dextran: 28.5\% of 40,000-mol wt dextran yeilded the 10\% lightest of young cells, and 30.5\% dextran provided the 10\% heaviest or old erythrocytes. Both normal and diabetic erythrocytes contain increased amounts of Hb AIa+b and Hb AIc in old as compared to young cells. In normal subjects, young cells contained 1.2+/-0.2\%, and old cells contained 1.8+/-0.4\% Hb AIa+b. Corresponding values for diabetic cells were 1.7+/-0.6 and 2.6+/-0.9\%. Hb AIc increased from 3.1+/-0.8 to 6.0+/-1.1\% in normals and from 5.1+/-2.1 to 10.1+/-3.7\% in diabetics. The results indicate that both cell age and diabetes are significant determinants of the amounts of Hb AIa+b and Hb AIc.
This article was published in J Clin Invest
and referenced in Journal of Diabetes & Metabolism