Author(s): MoralesPrieto DM, Schleussner E, Markert UR
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Abstract PROBLEM: Starting from the peri-implantation period, leukemia inhibitory factor (LIF) is a major regulator of trophoblast functions. Micro-RNAs (miRNA) are short non-coding RNA sequences, which regulate expression of genes at post-transcriptional level. The influence of LIF on miRNA expression in trophoblastic cells has not yet been analyzed and was focus of this investigation. METHOD OF STUDY: JEG-3 choriocarcinoma cells have been stimulated with LIF for 1, 2, 4, 6, and 24 hr. The expression of miR-9, miR-141, miR-21, miR-93, and let-7 g has been analyzed by real-time PCR. Subsequently, miR-141 has been silenced and over-expressed to test its role in the proliferation of JEG-3 cells after 24 and 48 hr. RESULTS: MiR-141 has been significantly downregulated by more than 50\% after LIF stimulation, while miR-21 and miR-93 expression has been significantly upregulated. Silencing of miR-141 completely inhibited the proliferation of JEG-3 cells, while over-expression had no effect. CONCLUSION: LIF regulates expression of miRNA in trophoblastic cells, which may be responsible for several functional effects induced by LIF. © 2011 John Wiley & Sons A/S.
This article was published in Am J Reprod Immunol
and referenced in Journal of Clinical Case Reports