alexa [Relationship between blast crisis of chronic myeloid leukemias and abnormality of p16 and calcitonin genes].
Molecular Biology

Molecular Biology

Journal of Cytology & Histology

Author(s): Xu D, Hong W, Wang S

Abstract Share this page

Abstract OBJECTIVE: To study the relationship between homozygous deletions of p16 gene and calcitonin hypermethylation and chronic myeloid leukemia (CML) blast crisis. METHODS: Semiquantitative multiplex polymerase chain reaction (PCR) was used to detect homozygous deletions of p16 gene and semiquantitative PCR to detect calcitonin gene hypermethylation in 53 CML patients. RESULTS: In 53 CML patients, homozygous deletion of p16 gene was found in none of them in chronic phase, while in 6.3\% (1/16) of them in myeloid blast crisis, 66.7\% (8/12) in lymphoid blast crisis, and 2/5 (40\%) in mixed cell blast crisis. Calcitonin gene hypermethylation was found in 10\% (2/20), 68.8\%(11/16), 16.7\%(2/12), and 40\% (2/5), respectively. CONCLUSION: There is intimate relationship between homozygous deletions of p16 gene and CML in lymphoid blast crisis, between calcitonin gene hypermethylation and CML in myeloid blast crisis. Moreover, abnormality of both genes may be associated with mixed cell blast crisis in CML patients. Simultaneous detection of the two genes help early pickup CML patients in blast crisis.
This article was published in Zhonghua Zhong Liu Za Zhi and referenced in Journal of Cytology & Histology

Relevant Expert PPTs

Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version