alexa Removal of biofilm from endoscopes: evaluation of detergent efficiency.
Bioinformatics & Systems Biology

Bioinformatics & Systems Biology

Current Synthetic and Systems Biology

Author(s): Vickery K, Pajkos A, Cossart Y

Abstract Share this page

Abstract BACKGROUND: Biofilm consisting of bacteria enclosed in a matrix of exopolysaccharide (EPS) forms on many medical devices such as catheters and implants. Nosocomial infection is, thus, a newly recognized scenario of biofilm development. Biofilm removal by physical methods such as ultrasound and mechanical cleaning is reasonably effective but difficult to supervise in practice. Chemical methods are often ineffective because of biofilm resistance to biocides. In this study, we compared the efficiency of different detergents used in endoscope reprocessing. METHODS: Escherichia coli biofilm was generated on Teflon and medical grade PVC tubing under low flow conditions. Sections of biofilm covered tubing were washed using test detergents and biofilm removal was assessed by counting remaining adherent bacteria after washing and by scanning electron microscopy to qualitatively assess the amount and nature of the remaining biofilm. RESULTS: Control tubing developed a multilayered biofilm consisting of >10(5) bacterial cells/cm(2). Only Matrix (Whiteley Medical, Sydney, Australia) produced >4 log reduction in viable bacterial numbers. Matrix and Epizyme Rapid (3M Australia, Pymble, Australia) were able to remove up to 75\% and 60\% of the biofilm, respectively. CONCLUSIONS: Many commonly used enzymatic cleaners fail to reduce the viable bacterial load or remove the bacterial EPS. Cleaners with high enzyme activity, Epizyme Rapid, removed more biofilm but failed to reduce bacterial numbers more than 2 logs. The only cleaner containing no enzymes, Matrix, significantly reduced bacterial viability and residual bacterial EPS. This article was published in Am J Infect Control and referenced in Current Synthetic and Systems Biology

Relevant Expert PPTs

Relevant Speaker PPTs

Recommended Conferences

Relevant Topics

Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri, Food, Aqua and Veterinary Science Journals

Dr. Krish

1-702-714-7001 Extn: 9040

Clinical and Biochemistry Journals

Datta A

1-702-714-7001Extn: 9037

Business & Management Journals


1-702-714-7001Extn: 9042

Chemical Engineering and Chemistry Journals

Gabriel Shaw

1-702-714-7001 Extn: 9040

Earth & Environmental Sciences

Katie Wilson

1-702-714-7001Extn: 9042

Engineering Journals

James Franklin

1-702-714-7001Extn: 9042

General Science and Health care Journals

Andrea Jason

1-702-714-7001Extn: 9043

Genetics and Molecular Biology Journals

Anna Melissa

1-702-714-7001 Extn: 9006

Immunology & Microbiology Journals

David Gorantl

1-702-714-7001Extn: 9014

Informatics Journals

Stephanie Skinner

1-702-714-7001Extn: 9039

Material Sciences Journals

Rachle Green

1-702-714-7001Extn: 9039

Mathematics and Physics Journals

Jim Willison

1-702-714-7001 Extn: 9042

Medical Journals

Nimmi Anna

1-702-714-7001 Extn: 9038

Neuroscience & Psychology Journals

Nathan T

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

John Behannon

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

1-702-714-7001 Extn: 9042

© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version