alexa Resolving postglacial phylogeography using high-throughput sequencing.
Agri and Aquaculture

Agri and Aquaculture

Agrotechnology

Author(s): Emerson KJ, Merz CR, Catchen JM, Hohenlohe PA, Cresko WA,

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Abstract The distinction between model and nonmodel organisms is becoming increasingly blurred. High-throughput, second-generation sequencing approaches are being applied to organisms based on their interesting ecological, physiological, developmental, or evolutionary properties and not on the depth of genetic information available for them. Here, we illustrate this point using a low-cost, efficient technique to determine the fine-scale phylogenetic relationships among recently diverged populations in a species. This application of restriction site-associated DNA tags (RAD tags) reveals previously unresolved genetic structure and direction of evolution in the pitcher plant mosquito, Wyeomyia smithii, from a southern Appalachian Mountain refugium following recession of the Laurentide Ice Sheet at 22,000-19,000 B.P. The RAD tag method can be used to identify detailed patterns of phylogeography in any organism regardless of existing genomic data, and, more broadly, to identify incipient speciation and genome-wide variation in natural populations in general.
This article was published in Proc Natl Acad Sci U S A and referenced in Agrotechnology

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