Author(s): P ForlayFrick, Z B Nagy, J Fekete, A Kettrup, I Gebefugi
A simple and sensitive high-performance liquid chromatographic method for the determination of vancomycin in influenza-vaccine has been developed. The stationary phase was a Purospher RP-18e column (125 × 3.5 mm; 5 μm; Merck); the mobile phase consisted of acetonitrile-water-phosphoric acid (85%) (8.5 / 91.5 / 0.125 v/v/v %) and its pH was adjusted to pH 2.80 using distillated triethylamine immediately prior to use. Separation was achieved using a flow rate of 0.5 mL/min at ambient temperature. The vancomycin was detected at 230 nm. The retention time for vancomycin was 7.20 ± 0.20 min. The limit of detection was 30 ng/mL; the limit of quantification was found to be 100 ng/mL.