alexa Reverse phase-HPLC and HPTLC methods for determination of gemifloxacin mesylate in human plasma.
Chemical Engineering

Chemical Engineering

Journal of Chromatography & Separation Techniques

Author(s): Rote AR, Pingle SP

Abstract Share this page

Abstract Two simple, rapid, sensitive and economic chromatographic methods have been developed for determination of gemifloxacin mesylate in human plasma by using internal standard. First method depends on reverse phase high performance liquid chromatography. The plasma sample was extracted using chloroform:acetic acid (5.4:0.1, v/v). A concentration range from 30 to 600 ng/ml was used for calibration curve. The percent recovery of gemifloxacin mesylate was found to be 80.06-84.88. The mobile phase used consist of methanol:sodium acetate (1\%):ortho phosphoric acid (65:35:0.5, v/v/v) with pH 2.1 and flow rate 0.8 ml/min in isocratic mode. The separation was carried out by UV-detector at wavelength 263 nm. Second method depends on high performance thin layer chromatography. The plasma sample was extracted using chloroform:acetic acid (5.9:0.1, v/v). A concentration range from 50 to 600 ng/spot was used for calibration curve. The percent recovery of gemifloxacin mesylate was found to be 80.01-86.17. The mobile phase used consists of ethyl acetate:methanol:ammonia (8.0:4.0:3.0, v/v/v). Densitometric analysis was carried out at wavelength 254 nm. The R(f) values for gemifloxacin mesylate and linezolide were 0.33+/-0.03 and 0.69+/-0.03 respectively. The stability of gemifloxacin mesylate in plasma was confirmed during three freeze-thaw cycles (-20 degrees C), on bench during 12 h. The proposed method was validated statistically and by performing recovery study for determination of gemifloxacin mesylate in human plasma. This article was published in J Chromatogr B Analyt Technol Biomed Life Sci and referenced in Journal of Chromatography & Separation Techniques

Relevant Expert PPTs

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

[email protected]

1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

eng[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

 
© 2008- 2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords