Author(s): Vos PD, Goor M, Gillis M, Ley JD
Deoxyribonucleic acid (DNA)-ribosomal ribonucleic acid (rRNA) hybridizations were carried out between 23S 14C- or ³H-labelled rRNAs from type strs. of P. fluorescens, P. acidovorans, P. solanacearum and Xanthomonas campestris and DNA from one or more strs. of several taxonomically assigned or unassigned phytopathogenic P. spp. The strs. were assigned to one of the following rRNA branches: (i) the P. fluorescens branch (authentic pseudomonads), also containing P. agarici, P. amygdali, P. asplenii, P. caricapapayae, P. cichorii, P. corrugata, P. fuscovaginae, P. marginalis, P. meliae, and P. syringae group, P. tolaasii, P. viridiflava and "P. washingtoniae"; (ii) the P. solanacearum rRNA branch, also containing P. andropogonis, P. caryophylli, P. cepacia, P. gladioli, P. glumae, P. rubrisubalbicans and P. woodsii; (iii) the P. acidovorans rRNA branch, also containing "P. alboprecipitans" (syn. of P. avenae), P. pseudoalcaligenes ssp. citrulli, P. rubrilineans and "P. setariae"; and (iv) the Xanthomonas rRNA branch, containing X. campestris, P. betle, "P. gardneri", P. hibiscicola, "P. mangiferaeindicae", "P. viticola" and "P. vitiswoodrowii". P. flectens and P. cissicola definitely have to be removed from the genus Pseudomonas. P. cattleyae and "P. viridilivida" are heterogeneous and require additional examination.