alexa rK39 antigen for the diagnosis of visceral leishmaniasis by using human saliva.
Genetics & Molecular Biology

Genetics & Molecular Biology

Journal of Molecular Biomarkers & Diagnosis

Author(s): Vaish M, Singh OP, Chakravarty J, Sundar S

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Abstract The rK39 rapid immunochromatographic test (ICT) is now being widely used in the diagnosis of visceral leishmaniasis (VL) using serum. We evaluated the presence of anti-rK-39 antibody in human saliva being noninvasive to replace the invasive procedures of diagnosis. Enzyme-linked immunosorbent assay (ELISA) and ICT assays were performed in 300 subjects: 114-confirmed VL patients, 95 and 47 healthy controls from endemic and nonendemic regions, respectively, and 44 subjects with different diseases. Sensitivity in saliva was 83.3\% by ELISA and 82.5\% by ICT, compared with 100\% for both ICT and ELISA in serum. Specificity in saliva was 100\%, 90.5\%, and 88.6\% with ELISA, and 91.48\%, 91.57\%, and 84.06\% using ICT, in nonendemic, endemic, and different diseases, respectively. In serum, specificity was 97\%, 88.5\%, and 89\% by ELISA and 100\%, 94.7\%, and 95.5\% by ICT in nonendemic, endemic, and different diseases, respectively. Saliva is not suitable for diagnosis of VL because of low sensitivity.
This article was published in Am J Trop Med Hyg and referenced in Journal of Molecular Biomarkers & Diagnosis

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