alexa RNA aptamers for targeting mitochondria using a mitochondria-based SELEX method.


Virology & Mycology

Author(s): Tawaraya Y, Hyodo M, Ara MN, Yamada Y, Harashima H

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Abstract The use of mitochondria-based systematic evolution of ligands by exponential enrichment (SELEX) was explored. Mitochondria were isolated from rat liver and confirmed intact by respiratory control index. Isolated mitochondria and a 2'-F RNA random library were mixed and the bound RNAs collected. The counter selection was applied with nucleus and unbound RNAs were collected. After 7 rounds of selection, two sequences (Mitomer1 and Mitomer2) were verified to bind to mitochondria and the truncated Mitomer2 (short Mitomer2) showed better binding to isolated mitochondria than Mitomer1.
This article was published in Biol Pharm Bull and referenced in Virology & Mycology

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