alexa Routine expression profiling of microarray gene signatures in acute leukaemia by real-time PCR of human bone marrow.


Journal of Clinical & Experimental Pathology

Author(s): Sakhinia E, Faranghpour M, Liu Yin JA, Brady G, Hoyland JA,

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Abstract Cancer subtype diagnosis using microarray signatures has the potential to transform pathological diagnosis but the routine measurement of genes signatures remains difficult. Reverse transcription polymerase chain reaction (RT-PCR) measurement of Indicator genes for acute myeloid leukaemia (AML) and acute lymphoblastic leukaemia (ALL) was used to determine gene signatures. Bone marrow (BM) mononuclear cells were sorted into total, CD34(+) and CD34(-) fractions, and mRNAs globally amplified from each fraction using polyA PCR. The expression profile of the 17 top-ranked genes distinguishing AML and ALL were measured by RT-PCR in five ALL, 26 AML, 12 AML remission, four chronic myeloid leukaemia (CML) and nine morphologically normal BM samples. All but two of the genes measured showed similar expression in AML and ALL to that reported previously. Specifically, c-MYB (P
  • DOI: 10.1111/j.1365-2141.2005.05594.x
  • This article was published in Br J Haematol and referenced in Journal of Clinical & Experimental Pathology

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