alexa RP-HPLC pre-column derivatization for analysis of omeprazole, tinidazole, doxycycline and clarithromycin.
Chemical Engineering

Chemical Engineering

Journal of Chromatography & Separation Techniques

Author(s): Darwish KM, Salama I, Mostafa S, ElSadek M

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Abstract A validated, reliable and accurate reversed-phase high performance liquid chromatographic method using pre-column derivatization was adopted for the simultaneous determination of two ternary mixtures containing omeprazole, tinidazole and doxycycline hyclate or clarithromycin. Separation was achieved on a C18 column, through a gradient elution system using acetonitrile-methanol-water adjusted to pH = 6.60. Drugs were detected at 277 nm over concentration ranges of 1-112, 5-125, 2.5-550 and 2.5-100 µg/mL for omeprazole, tinidazole, doxycycline hyclate and clarithromycin, respectively. This is the first method that has isolated and identified clarithromycin derivative by infrared and mass spectroscopy. This method is the first study for the simultaneous determination of omeprazole, tinidazole, doxycycline hyclate and clarithromycin in combined mixtures and pharmaceutical formulations. This article was published in J Chromatogr Sci and referenced in Journal of Chromatography & Separation Techniques

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