Author(s): Pai PN, Rao GK, Srinivas B, Puranik S
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Abstract A simple reverse phase liquid chromatographic method has been developed and subsequently validated for simultaneous determination of tinidazole and diloxanide furoate. The separation was carried out using a mobile phase consisting of acetonitrile, methanol and 0.2 M potassium dihydrogen phosphate (pH 5) in the ratio 2:3:2.The column used was SS Wakosil-II C-18 with a flow rate of 1 ml/min and UV detection at 282 nm. The described method was linear over a concentration range of 10-70 μg/ml and 10-90 μg/ml for the assay of diloxanide furoate and tinidazole, respectively. The mean recovery was found to be 100-101\% for tinidazole and 97-103\% for diloxanide furoate when determined at three different levels.
This article was published in Indian J Pharm Sci
and referenced in Journal of Chromatography & Separation Techniques