alexa Salvia miltiorrhiza induces VEGF expression and regulates expression of VEGF receptors in osteoblastic cells.



Author(s): Wenden A, Yang Y, Chai L, Wong RW

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Abstract This study investigated in vitro whether Salvia Miltiorrhiza Bunge (SM) induces vascular endothelial growth factor (VEGF) expression and regulates expression of VEGF receptors 1 (VEGFR-1) and 2 (VEGFR-2) on osteoblasts. MC3T3-E1 cells were cultured with SM and VEGF at points 24, 48 and 72 h. A blank control was included. The mRNA expression of VEGF, VEGFR-1 and VEGFR-2 was examined using real-time polymerase chain reaction. VEGF protein expression was examined using enzyme linked immunosorbent assay. SM increased VEGF mRNA expression by 21\% at 24 h (p < 0.05), 5\% (p < 0.05) at 48 h and 74\% (p < 0.001) at 72 h, while external VEGF intervention decreased the internal VEGF expression by 51\% at 24 h (p < 0.001) and increased it by 126\% at 72 h (p < 0.001). SM increased VEGF protein at 72 h (p < 0.05). VEGFR-1 mRNA expression, in response to SM, decreased by 8\% (p < 0.05) at 72 h and decreased following VEGF at 24 and 72 h by 20\% (p < 0.001) and 15\%, respectively, (p < 0.001). VEGFR-2 mRNA expression increased following SM at 24 and 48 h by 25\% (p < 0.05) and 73\% (p < 0.05), respectively, and decreased at 72 h by 13\% (p < 0.05). It was concluded that SM modulated expression of VEGF and its receptors in MC3T3-E1 cells. Copyright © 2013 John Wiley & Sons, Ltd. This article was published in Phytother Res and referenced in Dentistry

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