Author(s): Cheung AL, Nishina K, Manna AC
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Abstract The 375-bp sarA open reading frame is driven by three promoters, P1, P3, and P2. Using gel shift and DNase I footprinting assays, we found that SarA binds to two 26-bp sequences and one 31-bp sequence within the P1 and P3 promoters, respectively. Together with the results of transcription analyses, our data indicate that SarA binds to its own promoter to down-regulate sarA expression.
This article was published in J Bacteriol
and referenced in Journal of Analytical & Bioanalytical Techniques