alexa Screening actinomycetes for extracellular peroxidase activity.
Environmental Sciences

Environmental Sciences

Journal of Bioremediation & Biodegradation

Author(s): Mercer DK, Iqbal M, Miller P, McCarthy AJ

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Abstract A diverse collection of actinomycete strains were screened for production of extracellular peroxidase activity by adapting a chemiluminescence analysis system developed for horseradish peroxidase-based enzyme-linked immunosorbent assay. Extracellular peroxidase activity was found to be common but quantitatively variable, and this rapid and sensitive screening system permitted identification of a small group of high-producing strains. A range of spectrophotometric assays were compared for the measurement of peroxidase activity in concentrated culture supernatants of two selected thermophilic streptomycetes. Of these, the peroxide-dependent oxidation of 2,4-dichlorophenol was identified as the most robust and reproducible assay for quantitative studies.
This article was published in Appl Environ Microbiol and referenced in Journal of Bioremediation & Biodegradation

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