alexa Screening of sugar converting enzymes using quantitative MALDI-ToF mass spectrometry.
Chemical Engineering

Chemical Engineering

Mass Spectrometry & Purification Techniques

Author(s): Bungert D, Bastian S, HeckmannPohl DM, Giffhorn F, Heinzle E,

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Abstract Quantitative matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-ToF MS) was applied for the screening of ten pyranose oxidase variants. Quantitative MALDI-ToF MS using isotopic labeled internal standards and ionic liquid matrices was performed using aliquots of enzyme reaction mixtures without prepurification steps. The results obtained were in good agreement with HPLC measurements. Analysis time was approx. 3.5 min for a five-fold determination. Thus, quantitative MALDI-ToF MS can be used as a tool for screening of sugar converting enzymes. This article was published in Biotechnol Lett and referenced in Mass Spectrometry & Purification Techniques

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