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Nutrition

Nutrition

Journal of Food & Industrial Microbiology

Author(s): VH Segtnan, T Isaksson

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Several techniques for measuring quality parameters in foods by the use of near infrared (NIR) technology have been reported. The aim of this experiment is to evaluate the main techniques in order to find the optimal measurement conditions for NIR analysis of carbohydrates in fluid food systems. Two different model systems were studied, each system containing 61 designed samples. The first system was designed to give scatter, and was based on a commercial orange juice. The other system was designed to be scatter-free, and was based on distilled water. To all samples were added the same total amounts of glucose, fructose and sucrose, and measured using the following NIR techniques: transmittance measurements in cuvettes, dry extract diffuse reflectance (DESIR), fibre-optic transflectance and fibre-optic transmittance. Calibration models were made by partial least squares regression in the spectral regions 780-2500 nm for DESIR measurements, 1100-1315nm for 10mm pathlengths and 1100-1880+2130-2350nm for 1mm pathlengths. The models were fully cross-validated. Optimal prediction errors (Root Mean Square Error of Prediction, Cross-Validated) for DESIR measurements ranged from 0.020 to 0.030% (w/w), while 1mm cuvette values ranged from 0.008 to 0.012%. For these techniques there were only small differences between juice and water samples. Using fibre-optics, 1mm transmittance gave values in the range 0.068-0.081% for juice samples and 0.022-0.066% for water samples, while 1mm transflectance gave 0.044-0.051% for juice samples and 0.045-0.078% for water samples. 10mm pathlengths provided substantially higher prediction errors than 1mm for all techniques investigated. From these results, two main conclusions can be drawn. First, when measuring off-line, direct transmittance measurements in cuvettes gave better prediction results than DESIR. Second, when using fibre-optics, transflectance gave lower prediction errors than transmittance for scattering samples, while transmittance performed better than transflectance for non-scattering samples.

This article was published in Journal of Near Infrared Spectroscopy and referenced in Journal of Food & Industrial Microbiology

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