alexa Selective diffusion barriers separate membrane compartments.
Molecular Biology

Molecular Biology

Journal of Cytology & Histology

Author(s): Bruckbauer A, Dunne PD, James P, Howes E, Zhou D,

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Abstract We have investigated exchange of molecules between different membrane domains on a highly compartmentalized cell, the spermatozoon. Using Alexa Fluor 555-cholera toxin B-subunit we have observed clustering of preexisting GM1 gangliosides which diffused across the anterior acrosome-equatorial segment interface but did not access the postacrosome. By contrast, single lipid and protein molecules readily exchanged between all three domains, although they diffused more slowly on nearing and crossing to the postacrosome. Thus, two types of diffusion interfaces are present on sperm heads, an "open" interface and a "mass filter" interface. The latter seems to be due to a protein-cytoskeleton network. Copyright 2010 Biophysical Society. Published by Elsevier Inc. All rights reserved.
This article was published in Biophys J and referenced in Journal of Cytology & Histology

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