alexa Selective enrichment of Ser- Thr-phosphorylated peptides in the presence of Ser- Thr-glycosylated peptides.


Journal of Clinical & Cellular Immunology

Author(s): Poot AJ, Ruijter E, Nuijens T, Dirksen EH, Heck AJ,

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Abstract Modification through beta-elimination has proven to be a reliable first step in the approach for enrichment of serine/threonine-phopshorylated (Ser-/Thr) peptides. However, under harsh basic conditions, Ser-/Thr-glycosylated peptides are susceptible to beta-elimination as well. Therefore, we have optimized these conditions to achieve a beta-elimination that is highly selective for phosphorylated peptides. This is the first report of selective beta-elimination and enrichment of phosphorylated peptides in the presence of glycosylated peptides. This article was published in Proteomics and referenced in Journal of Clinical & Cellular Immunology

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