alexa Selective isolation of live dead cells using contactless dielectrophoresis (cDEP).


Journal of Membrane Science & Technology

Author(s): Shafiee H, Sano MB, Henslee EA, Caldwell JL, Davalos RV

Abstract Share this page

Abstract Contactless dielectrophoresis (cDEP) is a recently developed method of cell manipulation in which the electrodes are physically isolated from the sample. Here we present two microfluidic devices capable of selectively isolating live human leukemia cells from dead cells utilizing their electrical signatures. The effect of different voltages and frequencies on the gradient of the electric field and device performance was investigated numerically and validated experimentally. With these prototype devices we were able to achieve greater than 95\% removal efficiency at 0.2-0.5 mm s(-1) with 100\% selectivity between live and dead cells. In conjunction with enrichment, cDEP could be integrated with other technologies to yield fully automated lab-on-a-chip systems capable of sensing, sorting, and identifying rare cells. This article was published in Lab Chip and referenced in Journal of Membrane Science & Technology

Relevant Expert PPTs

Relevant Speaker PPTs

Recommended Conferences

  • 2nd International Conference and Expo on Water Microbiology & Novel Technologies
    (10 Plenary Forums 3 days 1 Event)
    August 28-30, 2017, Philadelphia, USA

Relevant Topics

Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version