alexa Selective isolation of young cells from human corneal endothelium by the sphere-forming assay.
Molecular Biology

Molecular Biology

Journal of Cell Science & Therapy

Author(s): Mimura T, Yamagami S, Yokoo S, Usui T, Amano S

Abstract Share this page

Abstract This study aimed to investigate whether a representative adult stem cell/precursor cell isolation method (the sphere-forming assay) could isolate cells with differences of telomere length, telomerase activity, and characteristics reflecting senescence. The sphere-forming assay was performed to obtain precursors from cultured sixth passage (P6) human corneal endothelial cells (CECs). P6 and P7 cultured CECs were used as the controls. Telomere length, telomerase activity, and senescence-associated factors were evaluated in precursors and controls. Precursors obtained from the spheres had longer telomeres and higher telomerase activity than cultured P6 cells. Strong positive staining for senescence-associated beta-galactosidase activity was detected in P6 and P7 cultured CECs, whereas little or no staining was detected in the precursors within spheres obtained from P6-cultured CECs or their progeny. The progeny of spheres derived from cultured CECs were small regular cells that grew at a higher density and contained more 5-bromo-2'-deoxyuridine-incorporating cells compared with the parental cultured cells. These findings indicate that the sphere-forming assay enriches precursors with longer telomeres, higher telomerase activity, and younger progeny than the original cells. Thus, the sphere-forming assay may contribute to obtaining the young cells needed for regenerative medicine. This article was published in Tissue Eng Part C Methods and referenced in Journal of Cell Science & Therapy

Relevant Expert PPTs

Relevant Speaker PPTs

Recommended Conferences

Relevant Topics

Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version