alexa Sensitivity of a rapid immuno-chromatographic test for hepatitis C antibodies detection.
Materials Science

Materials Science

Journal of Nanomedicine & Nanotechnology

Author(s): Desbois D, Vaghefi P, Savary J, Dussaix E, RoqueAfonso AM

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Abstract BACKGROUND AND OBJECTIVES: Enzyme-linked immunoassays (ELISA) are the most widely used anti-hepatitis C virus (HCV) screening tests but simple, instrument and electricity-free screening tests have been developed with results available in a few minutes. METHODS: The sensitivity of a rapid immuno-chromatographic assay for the detection of anti-HCV antibodies was evaluated on 421 HCV RNA-positive samples from chronic carriers and compared with ELISA method. RESULTS: The sensitivity of the ELISA method was 99.3\% and the sensitivity of the rapid test was 95.5\%. False negative results were independent of HCV genotype, but were associated with human immunodeficiency virus (HIV)-positive status. Among HIV-negative people, sensitivities of the rapid test and the EIA assay were 99.2\% and 100\%, respectively. Whereas among HIV-positive people, sensitivities were 77.5\% and 96.3\%. CONCLUSIONS: The immuno-chromatographic test is rapid and simple, and could be used along with rapid anti-HIV determination, in settings with limited facilities or when rapid results are required. This article was published in J Clin Virol and referenced in Journal of Nanomedicine & Nanotechnology

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