alexa Separation of phenolic compounds by high-performance liquid chromatography with absorbance and fluorimetric detection.
Chemical Engineering

Chemical Engineering

Journal of Chromatography & Separation Techniques

Author(s): RodrguezDelgado MA, Malovan S, Prez JP, Borges T, Garca Montelongo FJ

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Abstract Phenolic compounds including phenolic aldehydes, acids and flavonoids are separated by high-performance liquid chromatography (HPLC) with analysis time shorter than described in the literature. The use of a fluorescence detector in series with absorbance detector allowed increasing selectivity and sensitivity for the determination of catechin, vanillic acid, syringic acid, epicatechin and trans-resveratrol in wine samples. An optimised sample preparation method using liquid-liquid extraction with diethyl ether at pH 2.0 was used. The optimised method was applied to analyse wine samples with good results.
This article was published in J Chromatogr A and referenced in Journal of Chromatography & Separation Techniques

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