alexa Sequence-specific and visual identification of the influenza virus NS gene by azobenzene-tethered bis-peptide nucleic acid.
Microbiology

Microbiology

Journal of Antivirals & Antiretrovirals

Author(s): Kaihatsu K, Sawada S, Nakamura S, Nakaya T, Yasunaga T,

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Abstract To rapidly and specifically identify highly virulent influenza virus strains, we prepared an azobenzene-tethered hairpin-type peptide nucleic acid, bisPNA-AZO, which has a complementary sequence against a highly conserved genomic RNA sequence within the ribonucleoprotein complex of the 2009 pandemic influenza A virus, H1N1 subtype. bisPNA-AZO recognizes the conserved virus genome sequence in a sequence-specific manner. Immobilization of bisPNA-AZO on a plate allowed capture of the target virus gene and the generation of a visual colour signal.
This article was published in PLoS One and referenced in Journal of Antivirals & Antiretrovirals

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