alexa Sex determination from blood and teeth by PCR amplification of the alphoid satellite family.
Dentistry

Dentistry

Dentistry

Author(s): Hanaoka Y, Minaguchi K

Abstract Share this page

Abstract Sex determination from blood and teeth by PCR amplification of the alphoid satellite family according to the method of Witt and Erickson was investigated. Amplification of the X-(131 bp) and Y-(172 bp) specific sequences in males and that of the X-specific sequence in females was satisfactory in almost all samples. However, of the 22 samples (blood: 5, dental pulp: 12, tooth hard tissue: 5), a false positive fragment was detected at 172 bp among the female samples (blood: 17, dental pulp: 30, tooth hard tissue: 23) as a result of silver staining. These bands were not amplified by the dual PCR method and were different from the Y-specific band. Subsequent restriction enzyme digestion (DdeI, HinfI, and NciI) of these bands resulted in different patterns from the typical Y-specific band. Furthermore, in five male samples of hard tooth tissue specimens (n = 14), a Y-specific band was detected as a weak band, and in two of these five samples, this weak specific band was not amplified by dual PCR in spite of the male origin of the samples. These results showed that this method is useful for sex determination, but that dual PCR and restriction analysis should be used together in practical applications.
This article was published in J Forensic Sci and referenced in Dentistry

Relevant Expert PPTs

Recommended Conferences

  • 39th Asia-Pacific Dental and Oral Care Congress
    October 26-28, 2017 Osaka, Japan
  • American World Dentistry
    November 13-14, 2017 San Antonio, U
  • 39th South American Dental Congress
    Dec 4-6,2017 Sao Paulo,Brazil

Relevant Topics

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri, Food, Aqua and Veterinary Science Journals

Dr. Krish

[email protected]

1-702-714-7001 Extn: 9040

Clinical and Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemical Engineering and Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001 Extn: 9040

Earth & Environmental Sciences

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

General Science and Health care Journals

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics and Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001 Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Informatics Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Material Sciences Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Mathematics and Physics Journals

Jim Willison

[email protected]

1-702-714-7001 Extn: 9042

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001 Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

John Behannon

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001 Extn: 9042

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords