alexa Shift of whispering-gallery modes in microspheres by protein adsorption
Engineering

Engineering

Biosensors Journal

Author(s): S Holler, F Vollmer

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Biosensors based on the shift of whispering-galler y modes in microspheres accompanying protein adsorption are described by use of a perturbation theor y. For random spatial adsorption, theor y predicts that the shift should be inversely proportional to micorsphere radius R and proportional to protein surface density and excess polarizability. Measurements are found to be consistent with the theor y, and the correspondence enables the average surface area occupied by a single protein to be estimated. These results are consistent with cr ystallographic data for bovine serum albumin. The theoretical shift for adsorption of a single protein is found to be extremely sensitive to the target region, with adsorption in the most sensitive region var ying as 1  R 5  2 . Specif ic parameters for single protein or virus particle detection are predicted. © 2003 Optical Society of America

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This article was published in OPTICS LE TTERS and referenced in Biosensors Journal

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