alexa Simple high-performance liquid chromatographic assay for the determination of ciprofloxacin in human plasma with ultraviolet detection.


Pharmaceutical Analytical Chemistry

Author(s): Manuela Maya T, Nuno Gonalves J, Silva NB, Morais JA

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A simple high-performance liquid chromatographic method is described for the quantitative analysis of ciprofloxacin in human plasma. Following protein precipitation from plasma by means of 6% perchloric acid, the upper layer which contains the analyte and the internal standard lomefloxacin, was analysed on a reverse phase column LiChrospher 60 RP-select B (5 microm) (EcoCART 125-3) with ultraviolet detection at 280 nm. The mobile phase was acetic acid 5%-methanol-acetonitrile (90:5:5, v/v/v). The assay was linear for ciprofloxacin over the concentration range of 0.050 to 6.00 microg ml(-1). The limit of quantification (LOQ) was 0.050 microg ml(-1). The method was successfully applied to a bioavailability study with five different ciprofloxacin formulations.

This article was published in Journal of Chromatography B: Biomedical Sciences and Applications and referenced in Pharmaceutical Analytical Chemistry

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