Author(s): Hussain FN, Ajjan RA, Moustafa M, Anderson JC, Riley SA
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Abstract We describe a sensitive high-performance liquid chromatographic method for the determination of 5-aminosalicylic acid and N-acetyl-5-aminosalicylic acid in rectal tissue biopsies. Samples were derivatised using propionic anhydride and proteins were precipitated with methanol. A Supelcosil ABZ column (150x4.6 mm I.D., 5 microm silica particles) was used with a mobile phase comprising 0.1 M acetic acid, acetonitrile and triethylamine (1600:114:6, v/v/v). Fluorescence detection was employed and detection limits were 0.2 ng/mg tissue at a signal-to-noise ratio of three (measured concentration: 5-aminosalicylic acid, 0.254 (0.228-0.286) ng/mg, C.V. 10.7\%; N-acetyl-5-aminosalicylic acid, 0.18 (0.154-0.198) ng/mg, C.V 9.8\%). This assay was validated for use with serum, urine and faecal samples for which it proved to be both precise and accurate (C.V.<10\%, measured concentration within 10\%).
This article was published in J Chromatogr B Biomed Sci Appl
and referenced in Pharmaceutica Analytica Acta