Author(s): Laryea MD, Steinhagen F, Pawliczek S, Wendel U
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Abstract A simple and convenient method using commercially available derivatization reagents is described for the measurement of betaine and N,N-dimethylglycine (DMG) in blood and urine. Precolumn derivatization of plasma or urine is performed directly in acetonitrile without extraction with p-bromophenacyl bromide and crown ether as catalyst. The p-bromophenacyl ester derivatives are then separated by high-performance liquid chromatography, using an isocratic system of acetonitrile and water containing choline. Effluent was monitored at 254 nm. The limit of detection was 5 micromol/L for betaine and 2 micromol/L for DMG. Analytical recovery was >97\% for both analytes. Total and within-day CVs were 2.0-4.4\% and 0.9-2.2\% for DMG. For betaine, the total and within-day CVs were 1.3-5.3\% and 0.4-3.8\%, respectively. The method is precise and cost-effective and has been used successfully to determine the concentrations of DMG and betaine in human plasma and urine.
This article was published in Clin Chem
and referenced in Journal of Diabetes & Metabolism