Author(s): Meng QH, Gauthier D
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Abstract OBJECTIVES: To develop a HPLC method for the determination of citalopram (CIT) and desmethylcitalopram (DCIT). METHODS: Solid-phase extraction followed by fluorescence detection was used to measure CIT and DCIT in deproteinized plasma. RESULTS: The extraction recovery for both analytes was 104 +/- 3\%. The calibration was linear over the concentration range of 12-1600 ng/mL for CIT and 6-800 ng/mL for DCIT. The within-run CVs were 2.5\% for CIT (400 ng/mL) and 2.9\% for DCIT (200 ng/mL) and the between-run CVs were 5.2\% for CIT and 2.9\% for DCIT, respectively. With low concentrations of CIT (50 ng/mL) and DCIT (25 ng/mL), the within-run CVs were 3.1\% and 1.1\% and the between-run CVs were 7.4\% and 8.8\%, respectively. The lower limit of quantification was 12 ng/mL for CIT and 6 ng/mL for DCIT. CONCLUSIONS: This method allows for the simultaneous determination of CIT and DCIT in plasma at therapeutic and toxic drug concentrations.
This article was published in Clin Biochem
and referenced in Journal of Bioequivalence & Bioavailability