Author(s): Xiao Rong Yang, Chuang Xing Ye
Simultaneous analysis of purine alkaloids and catechins in tea from dry leaves of Camellia sinensis , Camellia ptilophylla and Camelliaassamica var. kucha by a reversed-phase high-performance liquid chromatography (RP-HPLC) method. This HPLC method had beenproved to be appropriate for the identiﬁcation and quantiﬁcation of purine alkaloids and catechins, and exhibited good correlation coef-ﬁcients, detection levels and recovery rates. Caﬀeine, theobromine, theacrine, (+)-catechin, ( À )-epicatechin, ( À )-epigallocatechin, ( À )-epigallocatechin gallate, ( À )-epicatechin gallate, ( À )-gallocatechin gallate and ( À )-gallocatechin were identiﬁed and quantiﬁed in thethree species of genus Camellia Sect. Thea . There was 2.72% caﬀeine and 0.26% theobromine in C. sinensis , 4.85% theobromine in C.ptilophylla , and 1.58% theacrine, 0.94% caﬀeine and 0.45% theobromine in C. assamica var. kucha. Theacrine in C. sinensis and C. ptil-ophylla , and caﬀeine in C. ptilophylla were not detected. These data highlight diﬀerences in the relative proportions of purine alkaloids inthe three species of Camellia Sect. Thea . In addition, diﬀerent catechins were identiﬁed and quantiﬁed. The highest content of catechin indry leaves was ( À )-epigallocatechin gallate (EGCG) 3.51%, ( À )-gallocatechin gallate (GCG) 9.88% and ( À )-epigallocatechin gallate(EGCG) 6.78% in C. sinensis , C. ptilophylla and C. assamica var. kucha , respectively.