alexa SIMULTANEOUS DETERMINATION OF METFORMIN AND PIOGLITAZONE BY REVERSED PHASE HPLC IN PHARMACEUTICAL DOSAGE FORMS
Chemical Engineering

Chemical Engineering

Journal of Chromatography & Separation Techniques

Author(s): KS LAKSHMI, T RAJESH, SHRINIVAS SHARMA

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A simple, sensitive and rapid reverse phase high performance liquid chromatographic method was developed for the estimation of Metformin Hcl (MET) and Pioglitazone (PIO) in pure and in pharmaceutical dosage forms. A Gemini C 18 column (150x4.6mm, 5μ) was used with a mobile phase containing a mixture of Acetonitrile and Ammonium Acetate buffer (pH-3) in the ratio of 42: 58. The flow rate was 0.3ml/min and effluents were monitored at 255nm and eluted at 5.17min (MET) and 8.1min (PIO). Calibration curve was plotted with a range from 0.5-50 μg/ml for MET and 0.3-30 μg/ml for PIO. The assay was validated for the parameters like accuracy, precision, robustness and system suitability parameters. The proposed method can be useful in the routine analysis for the determination on metformin and pioglitazone in pharmaceutical dosage forms.

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This article was published in I nternational Journal o f Pharmac y and Pharmaceutical Sciences and referenced in Journal of Chromatography & Separation Techniques

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